ATM Knockout cell line (22Rv1)

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ATM Knockout cell line (22Rv1) Order

Catalog#

YKO-H222

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ATM Knockout cell line（22RV1）

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:3

Species

Human

Gene Name

ATM

Gene ID

472

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% RPMI-1640+10% FBS

Accession

CVCL_D7FW(Cellosaurus)

Target region

Knockout region: Exon 3 (based on transcript ATM-229)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:22RV1			STR Info (Cell bank) Cell Line:22RV1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	12	13.0
D13S317	9	12		9	12
D7S820	9	10	11.0	10	11	9.0
D16S539	12	12		12	12
VWA	15	21		15	21
TH01	6	9.3		6	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	10	11		10	11
D12S391	18	25
FGA	20	23
D2S1338	17	18
D21S11	29	30
D18S51	13	14
D8S1179	13	14
D3S1358	14	15
D6S1043	16	16
PENTAE	5	13	14.0
D19S433	13	14
PENTAD	9	12
D1S1656	17	18	19.0

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 22RV1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATM gene knockout ATM Knockout cell line（22RV1） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ATM Knockout cell line (22Rv1)

Catalog#

YKO-H222

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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ATM Knockout cell line (22Rv1)

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